The restriction enzyme bamhi recognizes the dna sequence ggatcc and always cuts between the two g nucleotides. how many bases long is the sticky end of a dna molecule that has been cut with bamhi?

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03-12-2018
Biology

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The restriction enzyme bamhi recognizes the dna sequence ggatcc and always cuts between the two g nucleotides. how many bases long is the sticky end of a dna molecule that has been cut with bamhi?

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BioWizard BioWizard · Answer 1 · 2018-12-12T14:13:09+01:00

The correct answer is four base pairs.

BamHI is endonuclease meaning that it has the ability to cut DNA at specific target site. The recognition sequence of BamHI is 5'-GGATCC-3 and it cleaves it after the first G. Sticky ends are formed as a result of that cleavage remaining a four-base 5' overhang in one molecule and a complementary 5' overhang in the other. These ends are can be joined back together by an enzyme called ligase.