Answer:
Explanation:
Optical density, measured with a spectrophotometer, can be used to measure the concentration of bacteria in a suspension culture. Generally for bacterial cell culture OD reading at 600nm is measured. TYpically a bacterial cell culture of OD600 of 1.0 gives 8 x 108 cells/ml.
1) For culture A- OD value is 3.1, which represent vary dense E.coli culture, therefore should be diluted to keep the absorbance below 1.0 at 600nm.
For culture B-OD value of 0.03 is, which represent presence of very low aount of bacterial cells in the culture. So culture B further need to grow in specific condiiton to reach the OD value near about 1.0
