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An open tubular gas chromatography column is 35.7 m long and has an inner diameter of 0.250 mm. It is coated on the inside wall with a layer of stationary phase that is 1.8 µm (0.0018 mm) thick. Unretained solute, methane, passes through in 6.39 min, whereas toluene has a retention time of 16.35 min. Calculate the retention factor for toluene.

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jamesesther1234

Answer:

The retention factor, k is 2.49

Explanation:

According to the theory of High-Performance Liquid Chromatography (HPLC), the retention factor (or capacity), k, of a column is the ratio of the retention time of a retained analyte (toluene) to that of the  un-retained solute (methane).

This implies that:

k = [tex]\frac{16.35 (mins)}{ 6.39 (mins)}[/tex]  =  [tex]\frac{995 (secs)}{399 (secs)}[/tex]

 retention factor, k  = 2.49.

Note that there is no unit for retention factor, as it is a ratio.

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