Affinity chromatography is a method used to separate molecules with high affinity. This technique enables the purification of specific molecules ranging form nucleic acids, enzymes, antigens, antibodies, etc. In the example, the ligand will produce the receptor to be displaced from the beads and then eluted out.
Answer:
B: Molecules of purified receptor
Explanation:
Affinity chromatography is used mostly for biological systems because of high natural specificity between different biomolecules. Various applications include the use of enzymes, coenzymes, hormones, nucleotides, sugars etc to isolate viruses, cells and macromolecules. It has been widely applied to the purification of monoclonal antibodies, receptor proteins and recombinant proteins
Affinity chromatography is used to perform high levels of purification based on interaction between proteins and ligands. The ligand is attached to the solid matrix (chromatograph) by covalent bond, then a mixture of molecules is passed through the column. Only the molecules that show a higher affinity for the ligand would remain in the column while other materials that lacks specificity for the ligand would pass through the column. The molecules are further purified and reversibly adsorbed by an immobilised ligand. Successful binding interactions between the ligand and the protein must be reversible to avoid disruption of the biological properties during elution from the column.
