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Describe what changes you would expect to see in a plot of absorbance versus temperature in an optical melting experiment on a self-complementary duplex.

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Answer:

#Check explanation below for a detailed description.

Explanation:

A plot of absorbance vs temperature is used to determine  [tex]T_m[/tex] of a duplex DNA.

-The duplex DNA denatures.

-The denaturation creates two single strands which enhances Ultraviolet absorption ( increased temperatures increases absorption rates).

-Higher concentrations of [tex]Sodium \ Chloride[/tex] hampers stability of the DNA.

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