when using the crispr-cas9 system to make a mutation in a cell, the guide rna will contain which sequences (or complementary versions of the sequence):

Question

Queenjlife20 Queenjlife20

01-12-2022
Biology

contestada

when using the crispr-cas9 system to make a mutation in a cell, the guide rna will contain which sequences (or complementary versions of the sequence):

Respuesta :

Otras preguntas

what is database of computer ?

What's a Mudslinging. Please help?

Name the singer/songwriter of “On the 4th of July.”

According to marginal analysis, you should spend more time studying economics if the extra benefit from an additional hour of study: a. is positive. b. outweigh

What will be the next digit in the sentence 1, 1, 2, 3, 5, 8, 13, 21, 34?Please show me your way of thinking :)

what is the 14 the amendment

What special group advises the President of the United States? a. The Cabinet b. The Electoral College c. The Executive Branch

Which battle produced the bloodiest day of the Civil War?

In what city will you find NASA’s headquarters for all piloted U.S. space projects?

contexto1220 contexto1220 · Answer 1 · 2022-12-07T07:23:27+01:00

While using the crispr-cas9 system to make a mutation the sequence required is 20 bases sequence.

In an ordinary CRISPR study, an sgRNA is designed to have a manual collection domain (specific as gRNA in our study) on the 5′ end, that is complementary to the goal collection.

The rationally designed sgRNA is then used to manual the Cas9 protein to precise webweb sites withinside the genome for centered cleavage.Better concentrated on of CRISPR-Cas9In maximum instances the manual RNA includes a particular collection of 20 bases. These are complementary to the goal collection withinside the gene to be edited. However, now no longer all 20 bases want to suit for the manual RNA as a way to bind.